LoRR induced by 30% alcohol vapor was measured at six time points during the 2nd day of DD. (A) Mixed populations of male and female CS flies exhibited significant circadian rhythms in the LoRR (ANOVA: F5, 102 = 86.52, p < 0.001). (B) Complete course of LoRR during 1 h alcohol exposure shown for CT 9 and CT 21. (C) Circadian rhythm in 50% LoRR for male CS flies (ANOVA: F5, 48 = 53.55, p < 0.001) and (D) female CS flies (ANOVA: F5, 48 = 19.64, p < 0.001). (E) Comparison of time course of LoRR during 1 h alcohol exposure shown for males and females, CT 9 and CT 21. (F) Magnitude of the difference in LoRR between males and females in yoked experiments is phase-specific with the greatest difference between the sexes observed at CT 9 (ANOVA: F5, 48 = 6.23, p < 0.002).