FIGURE 3.

Ptpn22^−/− neutrophils are characterized by impaired immune receptor–induced ROS production. Neutrophils isolated from bone marrows of Ptpn22^−/− [knockout (ko)] and matched WT control mice were used to characterize ROS production following stimulation with PMA (A and B), with fMLF (C and D), or following plating of cells into wells coated with immobilized IC (IgG-BSA) or, as a control, BSA (E and F), into fibrinogen-coated wells in the presence (stimulated) or absence (control) of TNF-α (G and H), or into wells coated with the synthetic integrin ligand poly-Arg-Gly-Asp (polyRGD) or, as a control, heat-inactivated FCS (I and J). Data shown (means ± range) are representative individual experiments (A, C, E, G, and I). Total light emissions (means ± SEM) expressed as percentage of the response obtained with stimulated control neutrophils pooled from a minimum of three separate experiments (B, D, F, H, and J). *p < 0.05. RLU, relative light unit.