FIGURE 4.

Ptpn22^−/− neutrophils are characterized by reduced IC-induced degranulation. (A and B) Gelatinase release by control (wt) and Ptpn22^−/− [knockout (ko)] neutrophils plated into wells coated with immobilized ICs (IgG-BSA) or, as a control, BSA, or that had been stimulated by fMLF in the presence of cytochalasin B (fMLF + CB) was assessed by in-gel zymography. A representative experiment (A) and pooled data (means ± SEM) from five separately performed experiments (B) are presented. (C) Lactoferrin release by control and Ptpn22^−/− neutrophils plated into wells coated with immobilized ICs (IgG-BSA) or, as a control, BSA, or that had been stimulated by fMLF in the presence of cytochalasin B (fMLF + CB) was assayed by ELISA. Pooled data from three separate experiments are presented. Data presented are normalized to activated WT. *p < 0.05.