Figure 8.

Enhanced eosinophil differentiation in the absence of RhoH. Bone marrow was cultured in the presence of cytokines, and cell numbers and differentials determined every second day by counting and microscopic analysis of cytospins stained with Diff-Quik. Total number (a) and percentage (b) of eosinophils from bone marrow of WT and RhoH^−/− mice. (c) Mature eosinophils as percentage of total eosinophils in culture, demonstrating faster maturation of RhoH^−/− eosinophils. Results are representative of three independent experiments with 3–4 mice per group. (d) CCR3 and CD62L expression on in vitro differentiated eosinophils on days 12 and 14 as determined by flow cytometry. (e) GATA-1, PU.1, and GATA-2 mRNA expression in freshly isolated bone marrow (n=4). (f) Immunoblot analysis of GATA-2 protein expression in freshly isolated bone marrow. *P<0.05