Figure 5.

Rescue of male sterile phenotype by ectopic expression of wild-type DUBA in Duba¹⁰⁷ testes but not by inactive mutants. (a–c) Confocal projections of testes stained with DAPI (blue) and against HA-tag of transgenes (green), genotypes: (a) Bam-Gal4/Y; UAS-HA-Duba/+ Duba¹⁰⁷, (b) Bam-Gal4/Y; UAS-HA-Duba^C227A/+ Duba¹⁰⁷ and (c) Bam-Gal4/Y; UAS-HA-Duba^S183A/+ Duba¹⁰⁷. (d–f) Confocal projections of testes stained with DAPI (blue) and phalloidin (magenta), and antibodies against active caspase (cDcp-1, green) and polyglycylated tubulin (AXO49, red): (d) Bam-Gal4/Y; UAS-HA-Duba/+ Duba¹⁰⁷, (e) Bam-Gal4/Y; UAS-HA-Duba^C227A/+ Duba¹⁰⁷ and (f) Bam-Gal4/Y; UAS-HA-Duba^S183A/+ Duba¹⁰⁷. Scale bars: 50 μm (a–c) and 100 μm (d–f). The experiment was repeated two times. Three to six testes were investigated each time per condition