Figure 1.

Stox1 is expressed in granule neurons of EGL in the postnatal cerebellum. (a) Immunobloting of Stox1 in P0, P6, P14, P21 and adult mouse cerebellums shows that the expression level of Stox1 increases during the first 2 weeks after birth, but declines thereafter. (b) Immunobloting data show strong expression of Stox1 in EGL. Lane 1: GNPs, Lane 2: Purkinje cells and interneurons, Lane 3: spleen as negative control. (c) Dcx-positive and -negative granule cells from Dcx-DsRed mice were enriched using the flow cytometry sorting. Quantitative real-time PCR analysis shows significantly higher expression of Stox1, Dcx and DsRed and lower expression of Math1 in Dcx-positive granule cells compared with Dcx-negative GNPs. (n=6) (d) GNPs of Math1-GFP mice were collected for in vitro differentiation. The mRNA expression of Stox1 and Dcx increases along the 2 day differentiation. Scale bars=100 μm. (n=6). (***P<0.001, **P<0.01,*P<0.05, data are shown as means±S.E.M.)