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. 2016 Aug 26;23(12):2042–2053. doi: 10.1038/cdd.2016.85

Figure 3.

Figure 3

Figure 3

STOX1 overexpression promotes the differentiation of GNPs. (a) Quantitative RT-PCR and immunobloting analysis verifies the overexpression of Stox1 in Stox1 adenovirus-infected cells. (b) Infection of GNPs isolated from Math1-GFP mice with Stox1-expressing adenovirus leads to reduced GFP reporter expression. Flow cytometric analysis shows significantly decreased median fluorescence intensity of GFP in Stox1 overexpression GNPs (n=3). (c) Quantitative RT-PCR analysis shows that Stox1 overexpression results in downregulation of GNP marker Math1 and upregulation of the neuronal differentiation marker Dcx, Tuj1, Neurod1, NeuN. (d) Immunofluorescent staining indicates that Tuj1 expression is enhanced in Stox1 adenovirus-infected cerebellar granule cells. The scale bar represents 25 μm. (e) Immunofluorescent staining shows that overexpression of Stox1 in cerebellar granule cells increases the percentage of Dcx-positive cells in vitro. The scale bar represents 25 μm. (***P<0.001, **P<0.01,*P<0.05, data are shown as means±S.E.M.)