Figure 6.

Stox1 inhibits the tumor growth of SHH MB. (a) The mRNA level of Stox1 is significantly decreased in Patched^+/− mouse MB samples compared with cerebellar tissues from wild-type mice. (b) The Stox1 protein amount in Patched^+/− mouse MB samples is significantly lower than wild-type controls. (c) Cyclopamine treatment of Patched^+/− mouse MB cells results in enhanced transcription of Stox1 and repressed expression of Ccnd1 and Math1. (d) Stox1 overexpression significantly suppresses the xenograft growth of Patched^+/− MB cells (n=4). (e) Increased mRNA levels of Tuj1 and NeuN and decreased mRNA levels of Math1, Ccnd1 and Ccnd2 are detected in Patched^+/− MB cells infected with Stox1-expressing adenovirus. (***P<0.001, **P<0.01,*P<0.05, data are shown as means±S.E.M.)