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. 2016 Dec 5;7:1957. doi: 10.3389/fmicb.2016.01957

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Copyright © 2016 Ma, Lao, Jin, Lin, Cai and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Extracted ion chromatogram of the m/z 102 fragment (a characteristic of the homoserine lactone ring) of AHLs produced by representative strain (OTU2) recovered from preparative TLC. Identification of the AHL in the extract was determined from the mass spectrometry (MS) spectra by comparison with the corresponding AHL standards. For example, the MS data for the spot 1 (A) extract indicates the presence of a characteristic lactone fragment at m/z of 102 and the molecular ion peak at m/z of 200 or 220 [Na⁺], suggesting the AHL to be C6-AHL. Accordingly, The spot 2 (B), spot 3 (C), and spot 4 (D) are C6-O-, C8-, and (C10- or C12-) AHL, respectively.