Table 1.
N-acyl-homoserine lactone (AHL) profiling of 24 representative rhizobacteria isolated from the mangrove rhizosphere environment.
| Part A |
Part B |
|||||
|---|---|---|---|---|---|---|
| Strain No. | A136 | KYC55 | OTU No. | Closest cultivated bacteria | ID at16S-rRNA gene cocus | Potential AHL compounds∗ |
| Strain 1 | ++ | ++ | OTU1 | Enterobacter sp. | 99% | C12-, C8-OH, C6- |
| Strain 2 | ++ | ++ | OTU2 | Pasteurella pneumotropica | 100% | C12-, C10-, C8-OH, C6- |
| Strain 3 | ++ | ++ | OTU3 | Photobacterium rosenbergii | 99% | C12-, C8-, C8-OH |
| Strain 4 | ++ | ++ | OTU5 | Vibrio fluvialis | 99% | C8-OH |
| Strain 5 | ++ | ++ | OTU6 | Gallaecimonas sp. | 99% | C10- |
| Strain 6 | + | ++ | OTU8 | Staphylococcus xylosus | 99% | C10- |
| Strain 7 | + | + | OTU9 | Vibrio sinaloensis | 99% | C8-OH |
| Strain 8 | ++ | ++ | OTU10 | Brachybacterium paraconglomeratum | 99% | C14-, C12-, C8-OH |
| Strain 9 | + | + | OTU11 | Bacillus aerophilus | 99% | C12-, C10-, C8-OH |
| Strain 10 | ++ | ++ | OTU12 | Vibrio communis | 100% | C12-, C10-, C8-OH |
| Strain 11 | + | ++ | OTU13 | Staphylococcus saprophyticus | 100% | C14, C12-, C8-OH |
| Strain 12 | + | – | OTU14 | Photobacterium sp. | 99% | C12- |
| Strain 13 | + | + | OTU15 | Bacillus sp1. | 99% | C14-, C12- |
| Strain 14 | ++ | + | OTU17 | Bacillus aquimaris | 99% | C8- |
| Strain 15 | ++ | ++ | OTU18 | Vibrio sp. | 99% | C14-, C12-, C8-OH |
| Strain 16 | + | + | OTU19 | Bacillus cereus | 99% | C8- |
| Strain 17 | + | + | OTU20 | Paenibacillus sp. | 100% | C12-, C8- |
| Strain 18 | ++ | ++ | OTU21 | Paracoccus sp. | 100% | C12-, C10-, C8-, C6- |
| Strain 19 | ++ | ++ | OTU22 | Psychrobacter sp. | 99% | C14-, C12-, C8- |
| Strain 20 | + | + | OTU23 | Bacillus sp2. | 99% | C8- |
| Strain 21 | ++ | – | OTU24 | Alteromonas sp. | 99% | C10- |
| Strain 22 | ++ | ++ | OTU26 | Acinetobacter sp. | 99% | C12- |
| Strain 23 | ++ | ++ | OTU27 | Pseudomonas chlororaphis | 99% | C14-, C12-, C10- |
| Strain 24 | ++ | ++ | OTU28 | Pseudomonas aeruginosa | 98% | C14-, C10-, C8-OH |
++, Strong colouration; +, Weak colouration; -, No colouration. ∗Detection of AHL compounds using bioreporter strain A136 as an agar overlay on thin-layer chromatography (TLC) plates. AHL identified by the spot shape and corresponding Rf value. Part A: AHL activity detected from plate bioassays using bioreporters (A136 and KYC55). Part B: Identification and compounds information of AHL-producers.