Table 1.
Evaluation of five DNA markers and combinations of the markers.
| R | M | I | I2 | T | R+M | R+I | M+I | R+M+I | R+M+I2 | R+M+T | R+M+I+T | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Universality of primers | Yes | Yes | Yes | Yes | No | − | − | − | − | − | − | − |
| Rate of PCR success (%) | 100 | 100 | 100 | 100 | 100 | − | − | − | − | − | − | − |
| Rate of sequencing success (%) | 100 | 100 | 100 | 100 | 95.12 | − | − | − | − | − | − | − |
| Aligned length (bp) | 1187 | 815 | 827 | 269 | 1001 | 2002 | 2014 | 1642 | 2829 | 2271 | 3003 | 3830 |
| Average intra-distance (%) | 0.07 | 0.25 | 0.45 | 0.57 | 0.82 | 0.14 | 0.22 | 0.35 | 0.23 | 0.19 | 0.27 | 0.31 |
| Average inter-distance (%) | 0.57 | 1.90 | 6.74 | 9.92 | 2.25 | 1.14 | 3.08 | 4.21 | 2.70 | 2.08 | 1.33 | 2.63 |
| Average theta (Θ) (%) | 0.06 | 0.18 | 0.24 | 0.31 | 0.70 | 0.11 | 0.13 | 0.21 | 0.14 | 0.13 | 0.21 | 0.22 |
| Coalescent Depth (%) | 0.09 | 0.30 | 0.38 | 0.50 | 0.91 | 0.17 | 0.21 | 0.33 | 0.23 | 0.21 | 0.30 | 0.32 |
| Proportion of variable sites (%) | 5.48 | 19.63 | 37.12 | 52.42 | 10.49 | 11.24 | 18.42 | 28.44 | 18.77 | 16.12 | 10.76 | 16.42 |
| Proportion of parsimony sites (%) | 3.96 | 15.34 | 30.35 | 41.26 | 7.29 | 8.34 | 14.50 | 22.90 | 14.56 | 12.07 | 7.69 | 12.30 |
R, rbcL; M, matK; I, ITS; I2, ITS2; T, trnH-psbA.