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. 2016 Dec 5;2:16090. doi: 10.1038/cddiscovery.2016.90

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Copyright © 2016 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Characterization of porcine OTX2. (a) Phylogenetic tree of OTX2 protein calculated by online phylogenetics software. (b) Alignment of OTX2 protein sequences among animal species mouse (m), human (h), and pig (p). (c) Conserved domain of porcine OTX2 that retains a homeodomain sequence with 56 amino acids. Cd00086 denotes the accession number of the homeodomain sequence in the Conserved Domain Database. (d) Restriction enzyme digestions of pEGFP-OTX2 and pcDNA-OTX2. M, D2000 plus DNA ladder. (e) Western blotting analysis of GFP protein (27 kDa) and GFP-OTX2 fusion protein (65 kDa) in HEK-293 T cells. (f) GFP fluorescent assay. After a 36-h transfection, GFP- and GFP-OTX2-positive cells were observed by fluorescence microscopy. The inset is the enlarged image. Nuclei were stained with DAPI (blue). Scale bar, 50 μm.