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. 2016 Dec 5;2:16090. doi: 10.1038/cddiscovery.2016.90

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Copyright © 2016 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Overexpression of OTX2 in piPSCs. (a) Western blotting analysis of OTX2 protein expression and AP staining in OTX2 transfected PS23 cells. Scale bar, 50 μm. (b) Quantitative analysis of AP-positive colonies. (c) Quantitative RT-PCR analysis of pluripotent gene expressions in PS23 cells. OTX2+, piPS cells were transfected with pcDNA-OTX2; Ctrl, piPS cells without the pcDNA-OTX2 transfection. *P<0.05, **P<0.01, n=3.