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. 2016 Mar 10;92(7):487–491. doi: 10.1136/sextrans-2015-052475

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Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/

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Superimposed confocal laser scanning images with 400× magnification of Atopobium vaginae+Gardnerella vaginalis biofilm in six vaginal samples (A–F): vaginal epithelial cells DAPI in blue, A. vaginae-specific peptide nucleic acid (PNA)-probe AtoITM1 with Alexa Fluor 488 in green and G. vaginalis-specific PNA-probe Gard162 with Alexa Fluor 647 in red. For clarity, we omitted the BacUni-1 plane, such that the bacteria that did not hybridise with Gard162 and AtoITM1 are visible in DAPI blue only.