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. 2016 Feb 25;75(12):2150–2156. doi: 10.1136/annrheumdis-2015-208640

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This is an Open Access article distributed in accordance with the terms of the Creative Commons Attribution (CC BY 4.0) license, which permits others to distribute, remix, adapt and build upon this work, for commercial use, provided the original work is properly cited. See: http://creativecommons.org/licenses/by/4.0/

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The ankylosing spondylitis (AS)-risk allele at rs11209032 alters DNA-protein complex formation. Chemiluminescent electrophoretic mobility shift assay (EMSA) showing complex formation (i) after addition of HEK293 nuclear extract (lanes 2 and 7), and competition with 100-fold excess of unlabelled probes (lanes 3 and 8). TWIST1 antibody addition leads to a reduction or inhibition of the complex (i) (lanes 4 and 9, respectively). Addition of nonspecific IgG antibody (lanes 5 and 10). NE, nuclear extract; pro, protective. This result was confirmed in five independent experiments.