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. 2016 Feb 25;75(12):2150–2156. doi: 10.1136/annrheumdis-2015-208640

Figure 4.

Figure 4

Homozygosity for the ankylosing spondylitis (AS)-risk allele at rs11209032 is associated with reduced enhancer activity without altering mRNA levels of IL23R or IL12RB2. (A) H3K4Me1 methylation ChIP-qPCR assessed at the rs11209032 locus in CD4+ T cells (PMA and ionomycin-stimulated) from three ‘G/G’ and three ‘A/A’ patients. The fold enrichment is expressed as mean±SEM for each patient in triplicate. Positive control is IL10 enhancer. Student's t test was used. (B) The transcriptional activity of rs11209032 compared with minP (set to 1) was measured by luciferase reporter assays in HEK293T cells. The values of relative luciferase activity are expressed as mean±SEM of three or four repeat experiments each done in triplicate. One-way analysis of variance was used. (C) Relative amount of IL23R mRNA in primary CD4+ T-cells (PMA and ionomycin-stimulated) from 6 GG and 6 AA patients (normalised against β-actin). mRNA levels are expressed as mean±SEM. Student's t test was used. (D) Relative amount of IL12RB2 mRNA in primary CD4+ T-cells (PMA and ionomycin-stimulated) from 6 GG and 6 AA patients (normalised against β-actin). mRNA levels are expressed as mean±SEM. Student's t test was used. minP, minimal promoter.