Skip to main content
. 2016 Aug 24;71(12):1119–1129. doi: 10.1136/thoraxjnl-2015-208039

Figure 4.

Figure 4

Cytokine and protease expression and activity in lungs from mice exposed to nicotine-free or nicotine-containing e-cigarette fluids. Mice were exposed to inhaled nicotine-free (0 mg/mL) or to nicotine-containing (18 mg/mL) e-cigarette fluid for 4 months. Gene expression analysis by qPCR for (A) the cytokines IL-1β, MCP-1, CXCL10, IL-6, CXCL2 and CXCL5 and (B) proteases MMP-3, MMP-9, MMP-12, CatK, CatL1 and CatS. (C) Collagenase and cathepsin activity were measured in lung bronchoalveolar lavage fluid. Graphs are represented as mean±SEM of three measurements. p Values shown, comparing both treatments connected by a line. All n≥5 per group. The comparisons between two individual groups were determined by Student's t-test.