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Western blot analysis of Janus kinase 2 and signal transducer and activator of transcription 3 protein expression at 60 minutes in the control, high mobility group box 1 (HMGB1), AG490, and rapamycin groups. Beta-actin was used as the internal reference. Δ: p < 0.05 versus the control group; ▲: p < 0.01 versus the control group; ●: p < 0.01 versus the HMGB1 group.
