Figure 8. Protective effect from D-519 and D-520 in fly eyes expressing α-syn.

(A) Representative images of flies expressing α-syn^A30P through the Gal4-UAS system. Flies were fed the vehicle control (ultra pure water) or D-519 or D-520 (1 mg/mL in ultra-pure water) for 28 days. Flies were heterozygous for driver (GMR-Gal4), α-syn^A30Pand mCD8-GFP. (B) Quantification from fly heads shown in panel A and other flies that were fed vehicle control (ultra-pure water), D-519 or D-520 for the indicated amounts of time. *p < 0.05 based on one way ANOVA with Tukey’s post-hoc comparing flies fed the compound to same-day flies fed with vehicle control. (C) Representative images of fly eyes expressing α-syn and which were fed with either the vehicle control or the indicated compounds at different concentrations dissolved in ultra-pure water. (D) Quantification of eye fluorescence from α-syn-expressing flies that were fed vehicle control, D-519 or D-520 at the indicated concentration for 7 days after the adults emerged from their pupal cases. *p < 0.05 based on one way ANOVA with Tukey’s post-hoc correction comparing compound-fed flies to “ctrl” ones that were fed the vehicle control. Values are represented as means ± SD.