Skip to main content
. 2016 Dec 5;6:37926. doi: 10.1038/srep37926

Figure 6. Flow cytometric analyses of phagocytosis and oxidative burst in neutrophils of patients with cirrhosis.

Figure 6

Analysis of the neutrophils’ granulocyte functions by phagocytosis and oxidative burst in peripheral blood (A–H) and ascites (I–S). Neutrophils’ granulocytes were separated from peripheral blood (A,E) and ascites (I,O) by FSC vs. SSC and additionally characterised by surface staining with CD14 BV510 and CD16 V450 with (F,P) and without (B,K) stimulation with fluorescent E. coli. In peripheral blood, the expression of CD14 increased after stimulation (F) in contrast to neutrophils’ granulocytes in ascites (P), when both were compared to controls without stimulation (B,K). The analysis of phagocytosis and oxidative burst revealed a normal activity in peripheral blood’s neutrophils’ granulocytes (G,H), in contrast to a strongly attenuated activity in ascites’ neutrophils’ granulocytes (R,S), when both were compared to controls without stimulation (C,D,L,M). (A–D) Whole unstimulated blood. (E–H) Whole blood stimulated with fluorescent E. coli. (I–M) Ascites unstimulated. (O–S) Ascites stimulated with fluorescent E. coli.