Figure 5. Selection process of the subline from the SGC-7901 cell line.

Upper: the first round of selection. Parental cells were seeded into the cell channel. After 4 days, the cells that had invaded the open region (indicated by the arrow) were harvested and named SGC-7901/B1. Lower: the second round of selection. SGC-7901/B1 cells were re-seeded into the cell culture channel and subjected to the same selection. After 4 days of culture and invasion, the achieved subline cells used for amplification culture were named SGC-7901/B2 (original magnification ×100).