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. 2016 Dec 5;6:37637. doi: 10.1038/srep37637

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Comparative arrays of ED-iPSC lines A2.2.2, F3.5.2 and H3.3.1 showing representative images of differentiation to smooth muscle and cardiomyocytes, and compared to hESC H9 following protocols outlined in the text. Time frames shown all initiate from the pluripotent stage. Representative images are shown. (a) Smooth muscle. Cells are stained for Hoechst (blue), alpha smooth muscle actin (α-SMA, green). Acquired images show days 10, 14, 21, 28, and 35 of differentiation. (b) Cardiomyocytes. Expression of the cardiomyocyte-specific marker Troponin-T (green) is shown at days 14, 21, and 28. ED-iPSC lines with unpolymerized Troponin-T did not develop a contractile phenotype. (c) Differentiation of the H9 hESC pluripotent line into smooth muscle and cardiomyocytes. (d) Detailed differentiation protocol schematics, top one illustrates smooth muscle differentiation, while bottom one shows cardiomyocyte generation protocol. Scale bars are 50 μm.