Fig. 5.

LARGE2 can modify not only GPC4 but also other PGs. (A) Schematic representation of Fc-fusion constructs. Dotted arrow, suggested proteolytic cleavage site. Vertical lines, potential GAG attachment sites. (B) LARGE2 can modify PGs. Immunoblotting of Fc fusion proteins transiently expressed in the WT CHO cells with or without stable expression of LARGE2. (C) Immunoblotting of Fc-fusion proteins transiently expressed in HS- or GAG-deficient CHO mutant cells that stably express LARGE2. In the GAG-deficient cells, LARGE2 could not modify GPC4Fc or BGNFc.