Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Highly sensitive three-step western blotting. Purified LC20 (0.5–20 pg) was electrophoresed in a Laemmli SDS-gel and detected by 3-step western blotting with primary anti-LC20 antibody, secondary biotin-conjugated anti-IgG antibody and tertiary HRP-conjugated NeutrAvidin. As little as 0.5 pg of LC20 was detectable.
