Fig. 7.

Effects of the PKC inhibitor. Gö6983 (1 µmol/l), a broad PKC inhibitor, had no effect on ionomycin-induced contraction in both BCM (a) and guinea pig taenia caeci strips (b). It did not attenuate the inhibitory effect of okadaic acid in either case. (c) The observed tension was normalized to that of pre-contraction with ionomycin (white column). Treatment with Gö6983 alone did not cause significant change in either of the preparations (pale gray column, 101.0 ± 1.7%, P = 0.71 for BCM and 101.0 ± 2.7%, P = 0.86 for taenia caeci, vs. control). Okadaic acid (3 µmol/l) induced complete relaxation in the absence (dark gray column, 2.2 ± 0.7% for BCM and 2.2 ± 2.1% for taenia caeci) or presence (black column, 1.5 ± 2.6% for BCM and 2.8 ± 5.7% for taenia caeci) of Gö6983. Each column represents the mean ± SEM of 6 experiments. Statistical assessments by Student's t-test did not show significant difference in the force inhibiting effect of okadaic acid between the absence and presence of Gö6983 (P = 0.81 for BCM and P = 0.91 for taenia caeci).