Figure 3. Identification and quantification results.

(A) Proteins identified in the plasma MP from a non-infected mouse (NI) and a PbA-infected mouse at the stage of experimental cerebral malaria (ECM) (TMT⁰ experiment). The two samples shared 44% of the identifications. Only proteins identified with minimum 2 peptides and FDR ≤1% have been considered. (B) Comparison of the proteins identified in the pooled NI – d3 pi – ECM samples (TMT⁶#1 and TMT⁶#2 experiments) and those identified in the individual NI and ECM samples (TMT⁰ experiment). Globally, 368 murine plasma microparticle proteins have been identified. (C) Quantitative results obtained from the two TMT⁶ experiments. Proteins differentially abundant in MP were defined as having a p-value < 0.001 and a ratio ≥2.1 or ≤0.5. (D) Heat map showing the level of expression of the proteins found to be significantly differentially abundant for the three computed ratios, i.e. ECM/d3 pi, ECM/NI and d3 pi/NI, in the two quantitative experiments. nd = non differential proteins.