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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Protein Expr Purif. 2016 Feb 23;122:64–71. doi: 10.1016/j.pep.2016.02.012

Figure 3.

Figure 3

Coomassie stained SDS-PAGE of the purification process of MMP-8. (Lane 1) molecular weight standards, (lane 2) soluble fraction of the cell lysate, (lane 3) the flow through of application of the cell lysis to the Ni-IMAC, (lane 4) the flow through of a 40 mM wash of the Ni-IMAC, (lane 5) the gradient elution peaks from 40 to 500 mM imidazole, (lane 6) SEC separation of degradants from the gradient elution step, (lane 7) after incubating with thrombin for 12 hours, (lane 8) separation of thrombin cleavage fragments by SEC, (lane 9) after incubating with factor Xa for 16 hours, (lane 10) thrombin-cleaved thioredoxin tag separated by SEC.