Table 1.
Components of phage P22 and their masses.[28,32,39,42] The total phage mass was calculated by multiplying the mass of each component by its stoichiometry and summing all those masses together. For the genome the average DNA mass for the headful mechanism was used.30
| Component | Mass of one copy (Da) | Stoichiometry | Total Mass (Da) | |
|---|---|---|---|---|
| dsDNA | Genome + 1600 bp | 26,768,698 | 1 | 26,768,698 |
| Capsid | Coat protein, gp5 | 46,621 | 415 | 19,347,715 |
| Tail machine | Portal protein, gp1 | 82,612 | 12 | 2,889,621 |
| Plug protein, gp4 | 18,025 | 12 | ||
| Tailspike protein, gp9 | 71,857 | 18 | ||
| Plug protein, gp10 | 52,457 | 6 | ||
| Tail needle protein, gp26 | 24,603 | 3 | ||
| Ejection proteins | gp7 | 21,093 | (11) | 2,607,551 |
| gp16 | 64,358 | (12) | ||
| gp20 | 50,101 | (32) | ||
| Total Mass of Intact Virion (Da) | 51,613,585 | |||