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. Author manuscript; available in PMC: 2016 Dec 5.
Published in final edited form as: Curr Biol. 2002 Sep 3;12(17):1484–1495. doi: 10.1016/s0960-9822(02)01017-5

Figure 3. Requirement for CAF and HEN1 in miRNA Accumulation.

Figure 3

The accumulation of mature transcripts corresponding to MIR173, 163, 167, 159, 177, and 176 was undetectable in hen1-1 and caf-1 or was greatly reduced compared to wild-type. Note that MIR177 and MIR176 antisense probes detected an approximately 70-nt signal in all genotypes but detected a 27-nt signal only in wild-type. Lower exposures of the precursor-sized transcripts are shown on the right. The MIR178 panel is meant to show that the two transcripts of intermediate size (arrowheads) are not present in either hen1-1 or caf-1. The mature 20-nt transcript was not visible at this exposure. However, it was visibly present in wild-type but was absent from hen1-1 or caf-1 upon longer exposure. RNAs from the entire aerial portions of 1 month-old plants were used for the MIR173, 163, 167, and 159 hybridization, whereas RNAs from inflorescences were used for the MIR177, MIR176, and MIR178 hybridization. MW, molecular weight standards. Vertical arrows in MIR167 and MIR159 panels indicate signals from maize seedlings.