FIG 7 .

RasA interaction with Cdc42 is mediated by the IRD in A. fumigatus. (A) Lysates of the control strain and RasAΔIRD mutant were immunoprecipitated with anti-GFP agarose beads and subjected to Western blot analysis with either an anti-mCherry antibody (for mCherry-Cdc42 detection) or an anti-GFP antibody (for Ras protein detection). Images are from one representative experiment. Control experiments, including the inverse-pulldown reaction, are provided in Fig. S4 in the supplemental material. (B) Quantification of the amount of mCherry-Cdc42 immunoprecipitated with either RasA (from the control strain) or RasAIRD. Densitometry analysis was performed using ImageJ software, and the ratio of anti-mCherry signal to anti-GFP signal was calculated. The graph represents the average result from three separate experiments ± standard deviation. Data were compared using Student’s t test.