FIG 9 .

The endocytic rate is decreased in the RasAΔIRD mutant. Fluorescence micrographs showing FM4-64 uptake in a single hypha of the control strain (upper panels) and the RasAΔIRD mutant (lower panels) over 60 min at 37°C. AMM broth cultures of each strain were incubated at 37°C until approximately equal hyphal lengths were achieved for both strains. Cultures were then washed, loaded with 10 µM FM4-64 at 25°C, and further incubated at 37°C to monitor endocytic uptake of the dye. Images were acquired under identical exposure times. Note the delayed internal staining of RasAΔIRD hyphae (lower panels). Arrowheads denote locations of septa for both panels.