TABLE 2 .

Primers used in this study

Primer	Sequencea	Use
RasA ΔIRD For NotI	5′-TTTTGCGGCCGCATGGCTGAGTACAAGCTAGTTGTTG-3′	RasAΔIRD mutant
RasA Rev NotI	5′-TTTTGCGGCCGCTTACATAATAACGCATTTTCC-3′	All RasA mutants
ScIRD For NotI	5′-TTTTGCGGCCGCATGCCACTGAACAAGTCAAACATCCGGGAGTACAAGCTAGTTGTT-3′	N-terminal chimeras
CnIRD For NotI	5′-TTTTGCGGCCGCATGTCAAAGGCTCAATTCCTGCGGGAGTACAAGCTAGTTGTT-3′	N-terminal chimeras
NcIRD For NotI	5′-TTTTGCGGCCGCATGGCTAACAAGTTCACACGGGAGTACAAGCTAGTTGTT-3′	N-terminal chimeras
S3A For NotI	5′-TTTTGCGGCCGCATGGCTGCAAAGTTCCTTAGAGAGTACAAGC-3′	IRD mutation
K4A For NotI	5′-TTTTGCGGCCGCATGGCTTCAGCATTCCTTAGAGAGTACAAGC-3′	IRD mutation
F5A For NotI	5′-TTTTGCGGCCGCATGGCTTCAAAGGCACTTAGAGAGTACAAGC-3′	IRD mutation
L6A For NotI	5′-TTTTGCGGCCGCATGGCTTCAAAGTTCGCAAGAGAGTACAAGC-3′	IRD mutation
R7A For NotI	5′-TTTTGCGGCCGCATGGCTTCAAAGTTCCTTGCAGAGTACAAGC-3′	IRD mutation
R7K For NotI	5′-TTTTGCGGCCGCATGGCTTCAAAGTTCCTTAAAGAGTACAAGC-3′	IRD mutation
AfCdc42 For NotI	5′-TTTTGCGGCCGCATGGTGGTAGCTACAATT-3′	mCherry-Cdc42 fusion
AfCdc42 Rev NotI	5′-TTTTGCGGCCGCTTACAGCAAGACGCATCT-3′	mCherry-Cdc42 fusion

^aBoldface indicates nucleotides changed from the wild-type sequence to introduce the indicated mutation.