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. 2016 Aug 4;44(21):10117–10131. doi: 10.1093/nar/gkw692

Figure 2.

Figure 2.

CgpS functions as a silencer of CGP3 prophage activity. (A) Silencing of CGP3 induction. The prophage reporter strain C. glutamicum ATCC 13032::Plys-eyfp containing the cgpS overexpression plasmid pAN6-cgpS-Strep was cultivated in CGXII minimal medium in the presence or absence of IPTG (50 μM) and MMC (600 μM). The prophage reporter strain carrying the empty plasmid pAN6 served as a control. EYFP fluorescence was measured after 20 h of microplate cultivation. (B and C) CgpS is located in the nucleoid and displays colocalization with the phage adaptor protein AlpA (34). For co-localization studies, a C-terminal CgpS-mCherry fusion and a C-terminal fusion of the prophage adaptor protein AlpA to eYFP (pEC-XC99E) were analyzed. Both gene fusions were under control of the inducible tac promoter. DNA was stained with Hoechst Dye 33342. Fluorescence images were taken after 2.5 h of cultivation in CGXII with 150 μM IPTG. Scale bar, 2 μm.