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. 2016 Aug 4;44(21):10117–10131. doi: 10.1093/nar/gkw692

Figure 6.

Figure 6.

Impact of H-NS and Lsr2/Lsr2-like truncated variants on CGP3 prophage induction. (A and B) Impact of truncated CgpS orthologs on the growth and CGP3 prophage activity using the reporter strain ATCC 13032::Plys-eyfp. Shown is the cultivation in microtiter plates in CGXII minimal media with 25 μg·ml−1 kanamycin and 150 μM IPTG. As a control the reporter strain containing the empty plasmid was used. In (B), the fluorescence output after 20 h is shown in comparison to the uninduced samples. (C and D) Growth experiments were performed with WT::Plys-eyfp cells carrying hns on the overexpression plasmid pAN6 under the conditions described in (A). Expression of hns was induced with 50 μM IPTG. Fluorescence output of the prophage reporter after 20 h is shown in (D). (E) After 24 h, fluorescence images were taken of cells (of C and D) placed on agar pads. Scale bar is 2 μm. Data represent average values and standard deviations of three biological replicates.