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. 2016 Aug 26;44(21):e160. doi: 10.1093/nar/gkw744

Figure 5.

Figure 5.

DNA binding is protein- and environment-specific, (A–C) Raw survival distribution (black dots), the best fitting 2-component exponential decay (red line), and extracted distributions of diffusing, short and long binding events (pie chart) in Hht1p-HaloTag after (A) automated tracking filling in 1-frame gaps, (B) manual correction of the automated tracking in (A), and (C) automated tracking filling in gaps up to 4 frames. (D–F) Raw survival (black dots), best fit (red line), and distribution of binding events (pie chart) from automated tracking while filling in 4-frame gaps for (D) Ace1p-HaloTag, (E) Hsf1p-HaloTag in heat shock conditions, and (F) Hsf1p-HaloTag in absence of heat shock. For all distributions, the average residence times of the short and long binding events are included next to the appropriate slice with their 95% confidence interval. All distributions required a two-component exponential fit based on an F-test comparison between the one- and two-component fitting.