. 2016 Sep 20;44(21):10367–10376. doi: 10.1093/nar/gkw821

Table 1. Modes of integration of a mini-casposon encoding kanamycin resistance into pMA-Target catalyzed by tagged and de-tagged Cas1.

Enzyme	De-tagged Cas1		Tagged Cas1
Number of clones recovered	129 ± 34^a (2)^b		59 ± 19^a (4)^b
Total number of clones sequenced	9		28
Site of insertion	Insertion at the A. boonei target site	Insertion outside of the A. boonei target site	Insertion at the A. boonei TSD	Insertion outside of the A. boonei target site
Number of clones sequenced	4 clones	5 clones	26 clones	2 clones
Size of TSD at insertion site	15 bp (2 clones) 4 bp (2 clones)	16 bp (1 clone) 15 bp (1 clone) Unstable (1 clone) Deletion (2 clones)	15 bp (26 clones)	22 bp (1 clone) deletion (1 clone)

^anumber of clones ± standard deviation recovered per independent experiment.

^bnumber of independent experiments (insertion + transformation).

Following transformation and selection for resistance to kanamycin, transformants were picked randomly and the regions flanking the left and right TIR were sequenced to determine the site of integration and the extent of sequence duplication occurring at the target site.