Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Sep 30;44(21):10277–10291. doi: 10.1093/nar/gkw844

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Published by Oxford University Press on behalf of Nucleic Acids Research 2016. This work is written by (a) US Government employee(s) and is in the public domain in the US.

PMC Copyright notice

Figure 5. — (A) Alveolar development is dependent on the Stat5 autoregulatory enhancer. Histologically mammary tissue appeared normal in the presence of only one intact Stat5 allele (Stat5^+/−). Mammary tissue with homozygous deletion of the autoregulatory enhancer (Stat5^ΔE/ΔE) appeared less differentiated and Stat5^ΔE/− was severely underdeveloped. (B) Qualitatively, pSTAT5 staining of wild-type (Stat5^+/+) tissue appeared similar to Stat5^+/−. In contrast, pSTAT5 staining was greatly reduced in Stat5^ΔE/ΔE, Stat5^ΔE/− tissues. (C) Stat5a mRNA levels in wild-type and mutant mammary tissue (Stat5^+/+ n = 6; Stat5^+/- n = 2; Stat5^ΔE/ΔE n = 4; Stat5^ΔE/− n = 3). (D) The Western blot showed reduced STAT5 levels in Stat5^ΔE/ΔE tissue. GAPDH served as control, and showed no reduction. (E) Peak coverage in wild-type and mutant tissue. (F) STAT5A enhancers were reduced in Stat5^+/− tissue and completely absent in Stat5^ΔE/ΔE and Stat5^ΔE/− tissue. Reduction of H3K27ac was delayed in all genotypes except Stat5^ΔE/− where it was absent. (G) Representative genes with class 1, class 2 and class 3 enhancers. STAT5 binding in mutant tissue was most severely affected in class 1 enhancers (Glycam1) and the least in class 3 enhancers (Bcl6).