Figure 2.

(A) The initial fluorescence spectra of the Ce13d, NaA43 and 17E DNAzyme complexes with a 2AP replacing the rA_1.1 position in the absence of Na⁺. Relative fluorescence intensity at 370 nm of the 2AP-modified (B) Ce13d, (C) NaA43 and (D) 17E DNAzyme as a function of salt concentration. All the complexes were formed in buffer A (100 mM LiCl, 50 mM Tris, pH 7.5) with 1 μM 2AP-modified substrate strand and 2 μM enzyme. The dilution effect during titration was corrected. The error bars in all the figures represent the standard deviation from at least two independent measurements.