Figure 2.

Alignment of lsr promoter mutants versus wild type using Clustal Omega (37). Mutations are in red. Gray boxes highlight p-lsrR-boxes, CRPII, CRPI and p-lsrA-boxes. The methionine coded by the ATG start codon in the lsrR and lsrA gene, respectively are represented by solid arrows (←/→). (∗∗) indicates the location of the mutation found in both mutants, EP01rec and EP14rec sequences (G→A), and the mutation found in the EP08rec sequence (C→T) at the same location. Site-directed mutagenesis was performed on the region marked by the dashed arrows (<− −/− −>) in order to revert the mutations along the p-lsrR box (−237 to −197 upstream of the lsrA start codon).