Figure 4.
Growth of LW6 (ΔlsrR) (6) transformed with pLSR harboring the wild-type lsr operon promoter (Wt) and its mutants: EP01, EP14, EP01rec, EP14rec, E01recAG and EP14recAG in the presence of different concentrations of tetracycline. Serial dilutions of saturated cultures from 10−9 (rightmost) to 10−1 (leftmost) were spotted on OmniTray LB agar plates supplemented with with 34 μg/ml chloramphenicol and the indicated concentration of tetracycline. Growth imaged after 16 h of incubation at 37°C.