Table 2. Crystallization.

Method	Batch crystallization (salting out)
Container	Eppendorf microcentrifuge tubes (1.5 ml)
Temperature (K)	298
Protein concentration (mg ml−1)	10
Buffer composition of protein solution	10 mM HEPES, 300 mM NaCl pH 7.5
Composition of precipitant solution (initial condition)	0.1 M Tris–HCl pH 8.5, 0.2 M MgCl2, 22% PEG 4000