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. 2016 Nov 30;72(Pt 12):892–896. doi: 10.1107/S2053230X16017921

Table 1. Macromolecule-production information.

  Full-length FapF Truncated FapF
Source organism Pseudomonas PAO1 Pseudomonas UK4
DNA source Pseudomonas PAO1 gDNA pMMB190Ap:UK4fapA-F
Forward primer TACTTCCAATCCATGGCGACGGAATCCGAG GGCCGGTACCAAGGATGATTCGGAGCCGGC
Reverse primer TATCCACCTTTACTGTCAGAAGTAGTAGGGGAATTT CCGGAAGCTTTTAGAAGTAGTACGGGAATTTCAGGC
Cloning vector pNIC-NTH pRSF-1b
Expression vector pNIC-NTH pRSF-1b
Expression host E. coli BL21(DE3) E. coli Lemo21
Complete amino-acid sequence of the construct produced MHHHHHHHSSGVDLGTENLYFQSMATESEVEALKKELLELRQRYEAQQNALMVLEQRVRQVEAQPQAPQPQRLVKSIQPPAQARNDANAVAGTYGASLKDDGAPAPSVENIYQDASGFFGGGTFSLETGLTYSHYDTRQLFLNGFLALDSIFLGNIGVDQIDADIWTLDLTGRYNWNQRWQVDINAPVVYRESTYQSAGAGGSTSQITEKSVTGDPRLGDVSFGVAYKFLDESESTPDAVVSLRVKAPTGKDPYGIKLKQVPGNNNLNVPDDLPTGNGVWSITPGISLVKTVDPAVLFGSLSYTYNFEESFDDINPQQGVKTGGKVKLGNWFQLGVGVAFALNEKMSMSFSFSELISQKSKVKQDGQSWQTVSGSDANAGYFGLGMTYAVSNRFSIVPSLSIGITPDAPDFTFGVKFPYYF MKKTAIAIAVALAGFATVAQATSHHHHHHGTKDDSEPAQSVSNLYNEASGFFGNGKFSFETGITYARYDARQLTLNGFLALDSIFLGNINLDRIKADNWTLDLTGRYNLDNRWQFDVNVPVVYRESTYQSGGASGGDPQATSEESVSRDPTIGDVNFGIAYKFLDESATMPDAVVSVRVKAPTGKEPFGIKLVRSTANDNLYVPESLPTGNGVWSITPGLSLVKTFDPAVLFGSVSYTHNLEDSFDDISSDVNQKVGGKVRLGDSFQFGVGVAFALNERMSMSFSVSDLIQRKSKLKPDGGGWQSIVSSDANAGYFNVGMTIAASENLTIVPNLAIGMTDDAPDFTFSLKFPYYF