Table 2. Crystallization.

Method	Sitting-drop vapour diffusion
Plate type	24-well cell-culture plate
Temperature (K)	293
Protein concentration (mg ml−1)	10
Buffer composition of protein solution	20 mM Tris–HCl pH 8, 150 mM NaCl, 0.5% C8E4
Composition of reservoir solution	100 mM sodium citrate, 20–30%(w/v) PEG 400, 100 mM NaCl
Volume and ratio of drop	800 nl, 1:1
Volume of reservoir (µl)	80