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. 2016 Sep 19;17(11):1197–1205. doi: 10.1080/15384047.2016.1235668

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© 2016 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 5. — (A) Rb phosphorylation was assessed by immunoblotting in HT1080, MCF7, MDA-MB-231 and Hs578T cells. Phospho-Rb and unphospho-Rb are separated by differential migration on SDS-PAGE. (B) HT1080 cells expressing Dox-inducible PNUTS shRNA were grown in 3D cultures for 12 days, +/− Dox, followed by immunoblotting for PNUTS, Actin, and phospho-Rb and unphospho-Rb (indicated by arrows). Images are shown of cells +/− Dox stimulation. Scale bar, 200µm. (C) HT1080 cells grown in 2D cell culture were subjected to PNUTS depletion and immunoblotting was performed. The data shown is representative of 2 independent experiments.