Figure 7.

Reporter assays were performed using either WT or mutant E-cadherin promoter driven firefly luciferase expression constructs. proE-cad178-Luc (WT) contains the minimal region of the promoter required to exhibit activity and proE-cad178-Luc-mEbox (MUT) contains point mutations that abrogate promoter activity.⁴⁰ MDA-MB-231 cells were transfected with firefly luciferase constructs and Renilla luciferase as an internal vector control. Two days later cells were subjected to PNUTS depletion using PNUTS or NT (nontargeting) siRNA. Relative firefly/Renilla Luciferase activity was measured using the Dual Luciferase assay (Promega). Activity at the WT cadherin promoter in cells transfected with nontargeting siRNA was normalized to one. Experiment shown is representative of 5 independent experiments with n = 4. Error bars represent standard deviation of the mean.