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. 2016 Oct 7;17(11):1117–1125. doi: 10.1080/15384047.2016.1235654

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Figure 5. — hMOF expression is down-regulated in gastric cancer tissues. (A) hMOF expression level in gastric cancer tissue compared to normal tissue of the same case (n = 43). mRNA level of hMOF was measured with Q-PCR method. Cases with more than 2 folds of alteration were counted. (B) Bar plot summarization of hMOF transcription level between gastric normal and cancer tissues. (C) Upper panel: whole cellular hMOF protein levels and H4K16ac levels in 3 gastric cell lines. Lower panel: Relative hMOF mRNA levels of gastric cancer cells (SGC-7901 and MGC-803) normalized to somatic gastric epithermal cells (GES-1). (D) Upper panel: restoration of H4K16ac in MGC-803 cells by exogenous hMOF (Flag-tagged). Lower panel: cell viability of hMOF-transfected MGC-803 cells. GAPDH served as loading control. Asterisk: Significant difference (**: p < 0.01) compared to normal tissues/noncancerous cells/control plasmid treatment.