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MiR-4500 was downregulated in CRC cells and tissues. (A) The expression levels of miR-4500 were significantly reduced in CRC cells compared with FHC cells. Each bar represents the mean ± SD of 3 independent experiments. (B) The levels of miR-4500 were lower in tumors than in normal tissues; the data are presented as the mean ± SD (paired-samples t test). (C) Downregulated miR-4500 in CRC patients predicted poor overall survival. U6 was used as a loading control. ***P < 0.001.
