Figure 5.

HMGA2 was involved in miR-4500-regulated tumor growth in vitro and in vivo. (A) The expression of HMGA2 was examined by western blot. (B) The mRNA levels of HMGA2 were confirmed by RT-qPCR. The reintroduction of HMGA2 promoted cell growth and motility, as determined by MTT (B), flow cytometry (C), wound healing (D), and transwell (E) assays; scale bar: 100 µm. (F) HCT-116 cells (transfected with miR-4500 or mock) were subcutaneously injected into nude mice; tumors were harvested and weighed. (G) The expression of Ki-67 and HMGA2 in xenograft tumors was evaluated by IHC. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01.