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In vitro biotinylation of recombinant Msed ACC-β with recombinant Msed BPL. Purified M. sedula ACC-β and BPL were combined with biotin cofactor and incubated for 30 min at 70°C. This “reaction mixture” was applied to a streptavidin column and washed. Bound fraction consisting of biotinylated ACC-β was released from the beads by heat denaturation.
