Table II.
Recombinant M. sedula enzymes used in this study
Enzyme | Native Molecular Mass (kDa) |
Subunit Molecular Mass (kDa) |
Assembly | Comments |
---|---|---|---|---|
Acetyl-CoA carboxylase |
507 | α - 57 | (αβγ)4 | Agrees with native form (Hügler et al. 2003) |
β - 18.6 | ||||
γ - 57 | ||||
Carbonic anhydrase |
22 | 22 | α1 | Most β-CAs are heteromultimers, but Msed_0390 is monomeric |
Biotin protein ligase |
57 | 26 | α2 |
S. tokodaii homolog is monomeric (Li et al. 2006) |