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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Biotechnol Bioeng. 2016 Jun 30;113(12):2652–2660. doi: 10.1002/bit.26033

Table II.

Recombinant M. sedula enzymes used in this study

Enzyme Native
Molecular
Mass
(kDa)
Subunit
Molecular
Mass (kDa)
Assembly Comments
Acetyl-CoA
carboxylase
507 α - 57 (αβγ)4 Agrees with native form
(Hügler et al. 2003)
β - 18.6
γ - 57
Carbonic
anhydrase
22 22 α1 Most β-CAs are
heteromultimers, but
Msed_0390 is monomeric
Biotin protein
ligase
57 26 α2 S. tokodaii homolog is
monomeric (Li et al. 2006)